Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Internal Receptors01:31

Internal Receptors

69.4K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
69.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracranial Injection of Investigational Ex Vivo Expanded and Activated Gamma-Delta T Cells Engineered With a Methylguanine-DNA Methyltransferase-Expressing Lentivector in Patients With Primary Glioblastoma.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Non-Coding RNA-Based Therapeutic Strategies in Triple-Negative Breast Cancer: A Systematic Review.

International journal of molecular sciences·2026
Same author

Correction: Editorial: Current status and recent advances in preclinical models for rare cancers.

Frontiers in oncology·2025
Same author

Editorial: Current status and recent advances in preclinical models for rare cancers.

Frontiers in oncology·2025
Same author

Identification of Epigenetic Regulatory Networks of Gene Methylation-miRNA-Transcription Factor Feed-Forward Loops in Basal-like Breast Cancer.

Cells·2025
Same author

Integrative spatial omics reveals distinct tumor-promoting multicellular niches and immunosuppressive mechanisms in Black American and White American patients with TNBC.

Nature communications·2025

Related Experiment Video

Updated: Jun 3, 2025

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.1K

Dysregulated miRNA Expression and Androgen Receptor Loss in Racially Distinct Triple-Negative Breast Cancer.

Shristi Bhattarai1,2, Bruna M Sugita3, Emanuelle Nunes-Souza3

  • 1Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, GA 30144, USA.

International Journal of Molecular Sciences
|January 8, 2025
PubMed
Summary

Quadruple-negative breast cancer (QNBC) disproportionately affects African American women, linked to lower androgen receptor (AR) expression and poorer survival. Differentially expressed miRNAs may regulate AR, contributing to these racial disparities in QNBC.

Keywords:
androgen receptormiRNAprognosisquadruple negativeracetriple-negative breast cancer

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.8K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.7K

Related Experiment Videos

Last Updated: Jun 3, 2025

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.1K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.8K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.7K

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Triple-negative breast cancer (TNBC) lacking androgen receptor (AR) expression, termed quadruple-negative breast cancer (QNBC), disproportionately affects women of African descent, correlating with poorer overall survival (OS).
  • MicroRNAs (miRNAs) are key regulators of gene expression in TNBC, influencing critical signaling pathways, including AR signaling, with varying expression patterns across racial groups and cancer subtypes.
  • Understanding the molecular mechanisms underlying QNBC disparities is crucial for developing targeted therapies and improving outcomes for affected populations.

Purpose of the Study:

  • To investigate the role of differentially expressed miRNAs in regulating AR transcription in quadruple-negative breast cancer (QNBC).
  • To explore potential miRNA-mediated mechanisms contributing to the observed racial disparities in QNBC prevalence and prognosis between African American (AA) and European American (EA) women.
  • To identify specific miRNAs that target the AR gene and are differentially expressed in AA versus EA QNBC patients.

Main Methods:

  • Analysis of race-annotated TNBC patient samples (n = 129) to determine AR expression status and correlate with survival outcomes.
  • Utilizing The Cancer Genome Atlas (TCGA) RNA-seq data to compare AR mRNA levels between AA and EA patients with TNBC.
  • Employing bioinformatics to identify differentially expressed miRNAs between AA and EA QNBC patients and predict AR gene targets.

Main Results:

  • QNBC was more prevalent in African American (AA) patients (76.6%) compared to European American (EA) patients (57.7%), with AR loss significantly associated with poor survival in AAs.
  • AA patients with TNBC exhibited lower AR mRNA levels than EA patients in TCGA data, and AR-low AA patients had significantly poorer OS.
  • Forty-six miRNAs were differentially expressed between AA and EA QNBC patients, with ten specific miRNAs (miR-1185-5p, miR-1305, miR-3161, miR-3690, miR-494-3p, miR-509-3-5p, miR-619-3p, miR-628-3p, miR-873-5p, and miR-877-5p) predicted to target AR.

Conclusions:

  • Loss of AR expression is a significant factor linked to poorer prognoses in African American women with QNBC.
  • Specific differentially expressed miRNAs show potential as regulators of AR expression in QNBC, contributing to observed racial disparities.
  • Identifying these miRNA-AR regulatory pathways offers critical insights into the higher susceptibility of AA women to QNBC and may guide future therapeutic strategies.