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Related Experiment Video

Updated: Jun 12, 2026

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

Intestinal Cells-on-Chip for Permeability Studies.

Marit Keuper-Navis¹, Hossein Eslami Amirabadi^2,3, Joanne Donkers¹

¹Department of Metabolic Health Research, Netherlands Organisation for Applied Scientific Research (TNO), 2333 BE Leiden, The Netherlands.

|January 8, 2025

Summary

This summary is machine-generated.

A new intestine-on-chip system with a 3D-printed disc enables accurate measurement of intestinal permeability. This flexible model improves preclinical testing of drug compounds by mimicking the human small intestinal barrier.

Keywords:

cell monolayer in vitro model intestinal absorption intestinal barrier intestine-on-chip permeability

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Area of Science:

Biomedical Engineering
Drug Discovery
In Vitro Modeling

Background:

Accurate preclinical in vitro models are needed to assess intestinal permeability.
Existing intestine-on-chip systems require further optimization for specificity, integrity, and complexity.

Purpose of the Study:

To develop and validate a novel intestine-on-chip system for improved intestinal permeability studies.
To enhance preclinical testing platforms for drug compound evaluation.

Main Methods:

Adapted an established intestine-on-chip model for cell monolayer culture using a 3D-printed disc.
Investigated membrane characteristics and standardized read-outs under static and dynamic flow conditions.
Compared results to traditional static permeability studies.

Main Results:

The 3D-printed disc supported accurate Caco-2 and human enteroid monolayer formation.
The chip accurately detected barrier integrity and compound permeability under laminar flow.
Both flow dynamics and membrane properties significantly influenced permeability outcomes.

Conclusions:

The novel intestinal cell-on-chip system offers high flexibility for permeability studies.
Further validation with diverse compounds is necessary to realize the system's full potential.
This model advances preclinical assessment of the human small intestinal barrier.