Surface-Enhanced Raman Spectroscopy for the Detection of Reactive Oxygen Species

Reactive oxygen species (ROS) play fundamental roles in various biological and chemical processes in nature and industries, including cell signaling, disease development and aging, immune defenses, environmental remediation, pharmaceutical syntheses, metal corrosion, energy production, etc. As such, their detection is of paramount importance, but their accurate identification and quantification are technically challenging due to their transient nature with short lifetimes and low steady-state concentrations. As a highly sensitive and selective analytical technique, surface-enhanced Raman spectroscopy (SERS) is promising for detecting ROS in real-time, enabling in situ monitoring of ROS-involved electrochemical and biochemical events with exceptional resolution. This review provides a comprehensive analysis of the state-of-the-art in the SERS-based detection of ROS. Herein, the principles and ROS sensing mechanisms of SERS have been critically evaluated, highlighting their emerging applications in direct and indirect ROS monitoring in electrochemical and biological systems. The developments and reaction schemes of selective SERS probes for superoxide (^•O₂^-), hydroxyl radicals (^•OH), nitric oxide (^•NO), peroxynitrite (ONOO^-), and hypochlorite (OCl^-) are presented. Finally, technical challenges and future research directions are discussed to guide the design of SERS for ROS analysis.