Related Experiment Video
Updated: Jul 9, 2026
09:26
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Published on: October 15, 2013
14.0K
Relationship Between HSV-1 Serostatus and HSV-2 IgG Confirmation Results Using an Inhibition Assay.
Tessa M Shates1, Susan Realegeno, Harry E Prince
1Quest Diagnostics Inc., San Juan Capistrano, CA.
Sexually Transmitted Diseases
|January 8, 2025
Summary
Herpes Simplex Virus type 2 (HSV-2) IgG inhibition-indeterminate results are linked to higher HSV-1 IgG index values. This association highlights the need for further research into factors influencing indeterminate HSV-2 test results.
Area of Science:
- Immunology
- Virology
- Clinical Diagnostics
Background:
- A small percentage of herpes simplex virus type 2 (HSV-2) IgG enzyme immunoassay (screen assay) positive samples do not confirm via HSV-2 IgG inhibition assay.
- A subset of these non-confirming samples are indeterminate, showing inhibition with both HSV-1 and HSV-2 lysates.
Purpose of the Study:
- To investigate the association between HSV-1 IgG serostatus and HSV-2 IgG confirmation results using the HSV-2 IgG inhibition assay.
Main Methods:
- Consecutive HSV-2 IgG screen-positive specimens were analyzed.
- Specimens were tested for both HSV-1 IgG and HSV-2 IgG confirmation using the inhibition assay.
- The relationship between inhibition assay results and HSV-1 serostatus was evaluated.
Main Results:
- Of 21,006 HSV-2 IgG positive specimens, 13,237 (63%) were also HSV-1 IgG positive.
- HSV-1 IgG positivity was highest among inhibition-indeterminate samples (83%) compared to inhibition-negative (60%) and inhibition-positive (63%) samples.
- HSV-1 IgG-positive samples showed significantly higher median index values in the inhibition-indeterminate group compared to inhibition-negative and inhibition-positive groups.
Conclusions:
- HSV-2 IgG inhibition-indeterminate results are associated with elevated HSV-1 IgG index values.
- HSV-1 IgG serostatus does not appear to influence HSV-2 IgG inhibition-negative results.
- Additional research is required to elucidate other factors contributing to inhibition-indeterminate results.
Related Concept Videos
01:28Herpes
Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
01:23Genital Herpes
Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...