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Updated: Jun 3, 2025
Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
Published on: June 9, 2013
Diselenides as novel effective fluorescence quenchers to construct a two-photon fluorescent probe for thiols in a
1State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. zhangbx@lzu.edu.cn.
Abstract:
A fluorescence quenching mechanism using linear diselenides was proposed for the first time through a combination of intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET). Herein, we synthesized and screened a two-photon fluorescent probe AFC-SeSe, demonstrating a remarkable 300-fold increase in response to glutathione (GSH). Additionally, AFC-SeSe enabled real-time observation of increased thiol levels following treatment within a short timeframe in a mouse model of stroke.
