Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography

Avian influenza is a significant threat to the poultry industry, and it has become an outbreak in many countries because of its mortality and morbidity. Concerns about the history of avian influenza outbreaks has prompted all countries to enhance their independence in pharmaceutical and biological components as a preparedness measure for any potential occurrences. The production of antibodies such as IgY is a potential alternative. The potential of IgY biomaterials can be utilized as bioreceptors in diagnostic tools. This research aimed to purify IgY anti-avian influenza H5N1 produced from chicken eggs. IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated at 0.5 ml per bird dose using inactivated H5N1 vaccine with a purification stage from graded PEG, hydrophobic interaction chromatography, and concentrated using an ultrafilter. The present study shows that the IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated with an inactivated H5N1 vaccine, from 4 chicken eggs with a total protein content of 40.01 mg, it has biological activity with HI titer activity of 4,915.2 HI units and a recovery value of 24 % with hydrophobic interaction chromatography. Therefore, the purification process with hydrophobic interaction chromatography does not remove the IgY biological antibody. Further development will be required to perform antibody potency as a bioreceptor for diagnostic purposes.