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Updated: Jun 3, 2025

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
Published on: September 23, 2022
Proteomic Analysis of Mesenchymal Stem Cell-derived Exosomes in Psoriasis
Siyu Wang1, Qixin Han1, Jun Wang2
1Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Department of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Purpose:
This study aims to investigate the unique proteins in exosomes from mesenchymal stem cells derived from psoriatic lesions and compare them with those from healthy human skin. It seeks to identify potential regulatory factors that may influence the differential effects observed in these exosomes.
Methods:
Dermal mesenchymal stem cell exosomes were isolated from healthy human skin (HDMSCs-EXO) and psoriatic lesion of patient (PDMSCs-EXO). The extracted exosomes were analyzed through label-free quantitative proteomics to identify differential proteins. Bioinformatics analyses, including GO and KEGG enrichment, were conducted.
Results:
The comparative analysis using HDMSCs-EXO as a control group revealed 13 differential proteins. Notably, proteins such as B2R4D5, MFGE8, and MFAP5 are associated with the inflammatory mechanisms of psoriasis.
Conclusion:
The study identifies several differentially expressed proteins in exosomes may play roles in the development of psoriasis. These finding offer valuable insights for further exploration of the inflammatory processes in psoriasis.
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