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Electrochemical study of anti-platelets tirofiban HCl in dosage form using nanomaterials modified sensors: developed
Mohamed Rizk1, Maha Mahmoud Abou El-Alamin1, Ola Abd Elkhalek2
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Helwan University, P.O.Box 11795, Cairo, Egypt.
Insights
A new electrochemical method accurately quantifies tirofiban hydrochloride in pharmaceuticals. This method, using a modified carbon paste electrode, is crucial for treating heart failure and ensures drug quality.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Materials Science
Background:
- Tirofiban hydrochloride is vital for inhibiting platelet aggregation, impacting congestive heart failure treatment.
- Accurate quantification of tirofiban hydrochloride in pharmaceutical formulations is critical due to its role in managing a leading cause of death.
- Existing quantification methods may lack the sensitivity or efficiency required for pharmaceutical analysis.
Purpose of the Study:
- To develop and validate a novel electrochemical method for determining tirofiban hydrochloride in pharmaceutical dosage forms.
- To utilize a modified electrode for enhanced electrochemical detection of tirofiban hydrochloride.
- To assess the environmental impact and greenness of the developed analytical method.
Main Methods:
- Development of a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNT).
- Electrochemical determination of tirofiban hydrochloride using the modified electrode.
- Characterization of the electrode's morphology via Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray analysis (EDX).
- Validation of the method, including linearity, limit of detection, and correlation coefficient assessment.
- Evaluation of the method's greenness using the eco-scale and Green Analytical Procedure Index (GAPI).
Main Results:
- A linear electrochemical response for tirofiban hydrochloride was achieved in the range of 27.00-745.00 ng/mL.
- A high correlation coefficient of 0.9995 indicates excellent linearity.
- The method demonstrated a low detection limit of 15.50 ng/mL.
- Morphological analysis confirmed the successful modification of the carbon paste electrode with MWCNTs.
- The greenness profile of the method was successfully assessed.
Conclusions:
- The developed electrochemical method provides a sensitive and reliable approach for quantifying tirofiban hydrochloride in pharmaceutical formulations.
- The MWCNT-modified carbon paste electrode offers enhanced electrochemical performance for tirofiban hydrochloride detection.
- The method's green characteristics make it an environmentally friendly option for pharmaceutical quality control.
Abstract:
Tirofiban hydrochloride is used to inhibit platelet aggregation, which has a significant impact on the treatment of congestive heart failure the most common cause of death according to WHO. Therefore, its quantification in pharmaceutical dosage form is critical. In this work, an electrochemical method for the determination of tirofiban HCl in pharmaceutical dosage form was developed and validated. Carbon paste electrode modified with multi-walled carbon nanotubes (MWCNT) was utilized to examine the electrochemical response of tirofiban hydrochloride. Scanning electron microscopy and Energy-dispersive X-ray analysis was used to investigate the morphology of this electrode. A linear response was obtained within the range of (27.00-745.00 ng/mL) (5.4 × 10-5 M-1.5 × 10-3 M) with a correlation coefficient of 0.9995, and a detection limit of 15.50 ng/mL (3.1 × 10-5 M). The greenness profile of the method was assessed utilizing the eco-scale and the green analytical procedure Index.
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