Noval Selective Ligand-Extraction of Bioactive Components in Complex Natural Products Applying Immobilized...

Area of Science:

• Pharmacology
• Natural Product Chemistry
• Biochemistry

Background:

• Choerospondias axillaris is a traditional medicinal herb utilized for managing coronary heart disease (CHD).
• Its therapeutic effects are attributed to a wide range of anti-inflammatory properties.
• Understanding the active components and their mechanisms is crucial for validating its medicinal use.

Purpose of the Study:

• To identify and analyze the active anti-inflammatory components in Choerospondias axillaris.
• To investigate the pharmacodynamic basis of its efficacy in treating coronary heart disease.
• To develop an effective method for the enrichment and analysis of bioactive compounds in complex natural products.

Main Methods:

• Immobilization of Cyclooxygenase 2 (COX-2) and lipoxygenase 5 (5-LOX) enzymes on magnetic nanoparticles for selective ligand-extraction.
• Identification and analysis of extracted components using ultra-high-performance liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS/MS).
• Evaluation of the metabolization and pharmacodynamic effects of 16 identified components in rats with induced acute blood stasis.

Main Results:

• Sixteen active components were successfully extracted and identified, including notable flavonoids like Curcumin and Epicatechin.
• The study confirmed the presence and activity of these compounds, demonstrating their potential role in the anti-inflammatory effects of C. axillaris.
• Metabolization studies in rats provided evidence for a robust pharmacodynamic foundation for the identified compounds in the context of blood stasis.

Conclusions:

• The developed method enables effective enrichment, analysis, and efficacy evaluation of active components in complex natural products like C. axillaris.
• The identified compounds, particularly Curcumin and Epicatechin, contribute to the plant's established efficacy in treating coronary heart disease.
• This research provides a scientific basis for the traditional use of Choerospondias axillaris and offers a valuable approach for natural product research.