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Related Experiment Video

Updated: Jun 3, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

Pharmacokinetic analysis using single dilution assays: enhancing precision, reducing errors and increasing

Saloumeh K Fischer¹, Xiaome Xu², Hayeun Ji²

¹Bioanalytical Sciences (BAS), Genentech Research and Early Development, South San Francisco, CA, USA.

|January 9, 2025

Summary

This summary is machine-generated.

A novel assay using NUcleic acid Linked Immuno-Sandwich Assay (NULISA) technology offers a wide dynamic range for protein therapeutic quantification. This streamlines analysis, reduces errors, and improves throughput in clinical trials.

Keywords:

NULISA Pharmacokinetic automation dynamic range etrolizumab

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An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

Area of Science:

Biochemistry
Analytical Chemistry
Immunotechnology

Background:

Established methods like ELISA, MSD, and Gyrolab are used for protein therapeutic quantification in clinical trials.
These methods often have limited dynamic ranges, necessitating multiple sample dilutions.
Dilution steps can introduce errors and variability into assay results.

Purpose of the Study:

To develop and validate a novel assay for quantifying protein therapeutics with an improved dynamic range.
To assess the performance of the new assay compared to existing methods.

Main Methods:

Development of a pharmacokinetics assay utilizing the NUcleic acid Linked Immuno-Sandwich Assay (NULISA) platform.
Evaluation of the assay's concentration dynamic range and its performance with clinical samples.

Main Results:

The NULISA platform enabled the development of a pharmacokinetics assay with a concentration dynamic range exceeding 6 logs.
All clinical samples could be assessed with a single dilution, simplifying the process.
Results showed good correlation with established methods like ELISA and Gyrolab.

Conclusions:

NULISA technology provides high sensitivity, full automation, and a wide dynamic range for protein quantification.
This technology streamlines assay development, simplifies sample analysis, minimizes errors, and increases throughput.
NULISA represents a significant advancement for quantifying protein therapeutics in clinical settings.