Green fabricated bimetallic zinc ferrite nanoparticles mitigate oxidative stress-induced pathogenesis

Current study evaluates the beneficial role of bio-functionalized zinc ferrite nanoparticles fabricated from an aqueous extract of Decalepis hamiltonii leaves (DHLE.ZnFe₂O₄ NPs) on sodium nitrite (NaNO₂) and Diclofenac (DFC) induced oxidative stress in RBCs and Sprague Dawley male rat models. DHLE.ZnFe₂O₄ NPs were characterized using PXRD, FTIR, SEM-EDAX, HR-TEM and VSM. The data suggests that, DHLE.ZnFe₂O₄ NPs were crystalline, ellipsoidal in shape with an average size of 10.95 nm and super paramagnetic in nature. DHLE.ZnFe₂O₄ NPs exhibited anti-oxidant properties by scavenging DPPH, H₂O₂ and reducing ferric to ferrous ions. Furthermore, DHLE.ZnFe₂O₄ NPs normalized key parameters of oxidative stress such as LPO, PCC, TT and anti-oxidant enzymes (SOD & CAT). Similar to the previous in-vitro results, DHLE.ZnFe₂O₄ NPs restored all the said stress parameters in homogenates of the liver, kidney, pancreas and heart. In addition, DHLE.ZnFe₂O₄ NPs repaired Diclofenac induced tissue damage in the liver, kidney, pancreas and heart by regulating all biochemical parameters. Most importantly, DHLE.ZnFe₂O₄ NPs exhibited anti-inflammatory, anti-diabetic, anti-thrombotic activities and were non-toxic to RBCs. In conclusion, DHLE.ZnFe₂O₄ NPs through its anti-oxidant potential ameliorate oxidative stress induced pathogenesis such as, inflammation, tissue damage, diabetes and thrombosis.