Related Experiment Video

Updated: Jun 3, 2025

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

6.2K

Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT

Xiaofei Li1, Haibo Zhang1, Jianan Dong1

  • 1Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical University, No.12, Health Road, Shijiazhuang City, 050011, Hebei Province, China.

Scientific Reports
|January 9, 2025
PubMed

Insights

SHP2 tyrosine phosphatase promotes ovarian cancer progression by upregulating the integrin/E-Cadherin switch via ZEB1 signaling. E-Cadherin overexpression inhibits ovarian cancer cell migration and invasion, suggesting therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • SHP2 (PTPN11) is implicated in various cancers.
  • Its specific role and molecular mechanisms in ovarian cancer (OC) require further elucidation.

Purpose of the Study:

  • To investigate the function and molecular mechanisms of SHP2 in ovarian cancer.
  • To explore the relationship between SHP2, E-Cadherin, and cancer progression.

Main Methods:

  • Bioinformatics analysis of gene expression in OC.
  • Western blotting to detect protein expression (EGF, p-SHP2, ZEB1, E-Cadherin, etc.).
  • In vitro assays (cell migration, invasion) and in vivo tumor xenograft models in mice.

Main Results:

  • SHP2 and ZEB1 were highly expressed, while E-Cadherin was lowly expressed in OC tissues.
  • E-Cadherin overexpression reduced p-SHP2, ZEB1, and downstream signaling proteins, and inhibited SKOV3 cell migration and invasion.
  • SHP2 enhances OC cell motility and invasiveness by upregulating the integrin/E-Cadherin switch through ZEB1.

Conclusions:

  • SHP2 plays a crucial role in promoting ovarian cancer progression.
  • Targeting SHP2 or restoring E-Cadherin may represent a therapeutic strategy for ovarian cancer.
Keywords:
Eintegrin/E-Cadherin signalling pathwayEpithelial mesenchymal transitionOvarian cancerSHP2

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