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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Jun 3, 2025

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Published on: December 21, 2019

Modeling Malignant Mesothelioma in Genetically Engineered Mice.

Yuwaraj Kadariya¹, Eleonora Sementino¹, Xiang Hua²

¹Cancer Prevention and Control Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania.

Current Protocols

|January 10, 2025

Summary

This summary is machine-generated.

Mesothelioma research advances with genetically engineered mouse models (GEMMs) that mimic human disease, aiding the development of new cancer therapies and prevention strategies.

Keywords:

asbestos carcinogenicity conditional knockout mice intraperitoneal tumors intrapleural tumors mesothelioma

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Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

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Published on: May 2, 2022

Area of Science:

Oncology
Genetics
Toxicology

Background:

Mesothelioma is a rare but aggressive cancer linked to asbestos exposure and genetic mutations.
Key genetic factors include germline mutations in BAP1 and somatic mutations in BAP1, CDKN2A/B, and NF2.
Existing mouse models are crucial for understanding mesothelioma pathogenesis and testing interventions.

Purpose of the Study:

To present protocols for generating and utilizing genetically engineered mouse models (GEMMs) for mesothelioma research.
To detail methods for studying asbestos-induced mesothelioma and evaluating therapeutic strategies.
To highlight the clinical relevance of GEMMs in preclinical mesothelioma studies.

Main Methods:

Generation of GEMMs with germline or conditional knockout/knock-in alleles of key mesothelioma-associated genes (e.g., Bap1).
Asbestos exposure studies in GEMMs to investigate carcinogenicity.
Preclinical studies involving chemoprevention and chemotherapy in GEMMs with induced mesothelioma.

Main Results:

GEMMs closely recapitulate human mesothelioma's phenotype, genetic landscape, and inflammatory responses.
Asbestos-exposed mice with specific gene deletions show accelerated mesothelioma development.
Autochthonous GEMMs enable rapid tumor formation and therapeutic assessment in an immunocompetent setting.

Conclusions:

GEMMs provide invaluable tools for elucidating mesothelioma's biological underpinnings.
These models are essential for testing novel therapeutics, chemoprevention, and interception agents.
The presented protocols facilitate robust preclinical research for mesothelioma.