Protocol for applying Tumor Treating Fields in mouse models of cancer using the inovivo system

Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring. The inovivo system allows for the concurrent application of TTFields with standard cancer therapies. For complete details on the use and execution of this protocol, please refer to Barsheshet et al.¹.