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Updated: Jun 3, 2025

Lateral Root Inducible System in Arabidopsis and Maize
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miR166e/ZmATHB14 module contributes to drought tolerance in maize root.

Xiaotong Wei1, Chunlai Wang1, Yimeng Wang1

  • 1College of Agronomy, Jilin Agricultural University, Changchun 130118, China; Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

International Journal of Biological Macromolecules
|January 10, 2025
PubMed
Summary

Maize miR166e is crucial for drought tolerance. Knocking down miR166e enhances drought resistance by regulating the ZmATHB14 transcription factor, improving vascular development and ROS homeostasis.

Keywords:
DroughtMaizeROSVascular developmentmiR166

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of plant responses to environmental stresses.
  • Maize miR166 is a conserved miRNA involved in abiotic stress responses.
  • The specific role of miR166e in maize root drought stress response remains unclear.

Purpose of the Study:

  • To investigate the function of miR166e in maize root drought stress tolerance.
  • To identify the target gene(s) of miR166e involved in drought response.
  • To elucidate the regulatory mechanism of the miR166e-target gene module in drought tolerance.

Main Methods:

  • Quantitative real-time PCR to measure gene expression.
  • Genetic manipulation (knockdown and overexpression) of miR166e.
  • 5'-RACE and dual-luciferase assays to identify miRNA targets.
  • Analysis of ZmATHB14 expression and function under drought stress.
  • Liquid chromatography-mass spectrometry (HPLC-MS) for metabolic profiling.

Main Results:

  • Drought stress reduced the expression of the miR166e precursor in maize roots.
  • miR166e knockdown enhanced drought tolerance, while overexpression reduced it.
  • ZmATHB14, an HD-Zip III transcription factor, was identified as a direct target of miR166e.
  • ZmATHB14 positively regulates drought tolerance and its expression is inversely correlated with miR166e under drought.
  • The miR166e-ZmATHB14 module influences vascular development, ROS homeostasis, signaling pathways, and hormone levels.

Conclusions:

  • The miR166e-ZmATHB14 module plays a critical role in regulating maize root drought tolerance.
  • miR166e acts as a negative regulator, while its target ZmATHB14 acts as a positive regulator of drought tolerance.
  • This regulatory module impacts key physiological processes, including vascular development and oxidative stress management, under drought conditions.