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A----Z transition in the synthetic hexanucleotide (dCdGfl)3.

G V Fazakerley, S Uesugi, A Izumi

    FEBS Letters
    |March 25, 1985
    PubMed
    Summary
    This summary is machine-generated.

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    The hexanucleotide (dCdGfl)3 transitions between A-DNA and Z-DNA forms based on ionic strength. This conformational change, observed via NMR and CD spectroscopy, occurs at intermediate salt concentrations.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Spectroscopy

    Background:

    • DNA exists in various conformations, including A-DNA and Z-DNA.
    • Environmental factors like ionic strength can influence DNA structure.
    • Oligonucleotide sequences can exhibit distinct structural preferences.

    Purpose of the Study:

    • To investigate the conformational behavior of the hexanucleotide (dCdGfl)3.
    • To determine the effect of ionic strength on DNA structure.
    • To characterize the transition between different DNA forms.

    Main Methods:

    • Proton Nuclear Magnetic Resonance (NMR) spectroscopy at 500 MHz.
    • Nuclear Overhauser Effect (NOE) measurements.
    • Circular Dichroism (CD) spectroscopy.

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    Main Results:

    • At low ionic strength, (dCdGfl)3 adopts an A-DNA conformation.
    • At high salt concentrations, the hexanucleotide forms a Z-DNA structure.
    • A slow exchange between A-DNA and Z-DNA forms was observed at intermediate ionic strengths, confirmed by CD spectral changes.

    Conclusions:

    • Ionic strength is a critical determinant of DNA conformation for this hexanucleotide.
    • (dCdGfl)3 exhibits a salt-dependent switch between A-DNA and Z-DNA forms.
    • NMR and CD spectroscopy are effective tools for studying DNA conformational transitions.