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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Related Experiment Video

Updated: Jun 3, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

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Engineering an optimized hypercompact CRISPR/Cas12j-8 system for efficient genome editing in plants.

Shasha Bai1, Xingyu Cao1, Lizhe Hu1

  • 1Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, Inner Mongolia University, Hohhot, China.

Plant Biotechnology Journal
|January 12, 2025
PubMed
Summary
This summary is machine-generated.

Engineered CRISPR Cas12j-8 shows enhanced genome editing efficiency in plants. This improved system enables precise base editing and nuclease cleavage for crop improvement.

Keywords:
Cas12j‐8base editingcrRNAgenome editingplants

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Plant Science

Background:

  • The Cas12j-8 nuclease, a compact Type V CRISPR system component, offers potential for crop genome editing due to its small size and TTN PAM recognition.
  • However, its low editing efficiency in plants has limited its practical application.

Purpose of the Study:

  • To enhance the genome editing efficiency of the Cas12j-8 system in plants.
  • To develop a more robust and versatile CRISPR tool for plant biotechnology.

Main Methods:

  • Rational engineering of both the Cas12j-8 nuclease and its associated crRNA.
  • Testing the engineered system's editing activity in soybean and rice.
  • Development of cytosine base editors utilizing the engineered Cas12j-8 system.

Main Results:

  • Engineered Cas12j-8 and crRNA significantly improved genome editing efficiency in plants.
  • The combined system demonstrated robust activity in soybean and rice, editing previously inaccessible sites.
  • Editing efficiency in some cases matched or exceeded SpCas9 and outperformed the Cas12j-2 variant.
  • Developed base editors showed a 5.36- to 6.85-fold increase in base-editing efficiency (C to T) without indels.

Conclusions:

  • The engineered hypercompact CRISPR/Cas12j-8 system is a highly efficient tool for plant genome editing.
  • This enhanced system facilitates both nuclease-mediated cleavage and precise base editing in plants.
  • The findings pave the way for advanced crop improvement strategies using CRISPR technology.