Exploring the impact of cuproptosis-related genes on immune infiltration in rheumatoid arthritis
Fang Xu1,2, Xin-Ran Hu3, Yuan Wang3
1Graduate School of Dalian Medical University, Dalian, China.
Abstract:
Immune infiltration plays a significant role in the pathogenesis of rheumatoid arthritis (RA). Cuproptosis, a newly characterized form of programmed cell death, remains insufficiently investigated regarding its genetic regulation of immune infiltration in RA. Data from the GEO database were analyzed to determine the relationship between cuproptosis-related genes and immune infiltration. Comprehensive analyses, including Gene Ontology, and KEGG pathway enrichment were performed to construct a risk model and provide a theoretical framework for understanding the mechanisms underlying the involvement of cuproptosis in RA-related immune infiltration. And we explored the expression of related genes-PDHB in animal and cell experiments. The findings revealed substantial variations in immune infiltration between RA and control groups. Eleven cuproptosis-related genes associated with RA were identified, namely DLST, LIAS, DLAT, DLD, PDHB, Lipt1, DBT, ATP7B, SLC31A1, FDX1, and PDHA1. Among these, DLST and PDHB emerged as potential risk factors influencing RA pathogenesis. These genes modulated immune cell alterations, including changes in iDCs, NK-cells, mast cells, and pDCs, alongside impacts on immune functions specific to RA. In animal experiments, we found that the expression of PDHB is decreased; overexpression of PDHB by PDHB gene transfection inhibited the migration, invasion, and proliferation of FLS cells.
More Related Videos
11:03A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
Published on: October 30, 2019
09:08Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
Published on: June 2, 2021
Related Concept Videos
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
The JAK-STAT Signaling Pathway
Inflammatory Response
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Regulation of Hematopoietic Stem Cells
