Smart Bioelectronic Nanomesh Face Masks with Permeability and Flexibility for Monitoring Cortisol in Saliva

Bioelectronic face masks can easily collect biomarkers in saliva, in which free cortisol is abundant. However, conventional bioelectronic face masks involve significant challenges in terms of permeability and inhalation due to their nonpermeable film-type structure. Herein, we introduce a flexible and permeable nanomesh-based wearable biosensor designed for bioelectronic face masks that monitor cortisol levels. The diameter of the nanofiber matrix has a range of 200 to 500 nm and offers outstanding flexibility (2% resistance change at a bending radius of 2 mm), reliability (0.3% resistance change at a bending radius of 5 mm after 1000 bending cycles), and permeability (116.91 g m^-2 h^-1 at 18 °C with 40% humidity, which is 10 times higher compared with film) based on the nanoporous structure. We evaluated the electrochemical responses of functionalized interdigitated electrodes on a flexible and permeable poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) nanomesh. Our nanomesh cortisol biosensors demonstrated exceptional sensitivity to cortisol, even at low concentrations, with a detection limit as low as 10 pM. Furthermore, we measured cortisol in clinical samples, such as artificial saliva and human saliva, using nanomesh-based bioelectronic face masks. This study highlights the potential for further applications of bioelectronic face masks for detecting numerous biomarkers.