Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Iron dextran nanoparticles interacting with micrometric crosslinked protein scaffolds: Theoretical, computational and experimental results to design formulations for iron deficiency.

International journal of biological macromolecules·2025

Same author

Parenteral iron nutrition: Iron dextran-poloxamer thermosensitive hydrogel for prolonged intramuscular iron supplementation.

International journal of pharmaceutics·2024

Same author

Thermosensitive Chitosan Hydrogels: A Potential Strategy for Prolonged Iron Dextran Parenteral Supplementation.

Polymers·2024

Same author

Consistency across multi-omics layers in a drug-perturbed gut microbial community.

Molecular systems biology·2023

Same author

Leveraging interactions in microfluidic droplets for enhanced biotechnology screens.

Current opinion in biotechnology·2023

Same author

Open hardware: From DIY trend to global transformation in access to laboratory equipment.

PLoS biology·2023

Search research articles

Related Experiment Video

Updated: Jun 2, 2025

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

Plasmid Stability Analysis with Open-Source Droplet Microfluidics.

Pierre Padilla-Huamantinco¹, Emerson Durán², Tobias Wenzel³

¹Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile.

Journal of Visualized Experiments : Jove

|January 13, 2025

Summary

This study presents a microfluidic method for analyzing plasmid retention in single cells without antibiotics. The open-hardware workflow enhances accessibility for high-throughput biological analyses.

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Related Experiment Videos

Last Updated: Jun 2, 2025

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Area of Science:

Synthetic Biology
Microfluidics
Biotechnology

Background:

Plasmids are crucial for gene expression in synthetic biology.
Antibiotic selection for plasmid maintenance can be limiting.
Developing antibiotic-free methods for plasmid retention analysis is essential.

Purpose of the Study:

To develop an open-hardware microfluidic workflow for analyzing plasmid retention.
To enable high-throughput, antibiotic-free assessment of plasmid stability.
To provide a versatile platform for single-cell biological analysis.

Main Methods:

Utilizing droplet microfluidics for single-cell culture in gel microdroplets.
Employing fluorescence microscopy for quantifying microcolonies and plasmid retention.
Integrating open-source hardware for precise flow and temperature control.

Main Results:

Demonstrated parallel analysis of numerous single cells and microcolonies.
Enabled differentiation of cells based on plasmid loss using fluorescent markers.
Achieved enhanced statistical power compared to traditional methods.

Conclusions:

The developed microfluidic workflow offers a robust, accessible, and high-throughput method for analyzing plasmid retention.
Open-source hardware implementation increases the accessibility of advanced bioanalytical techniques.
The versatile platform can be adapted for diverse fluorescence-based single-cell studies.