Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ATG8 E3-like ligases sense lysosomal damage and initiate ESCRT-mediated membrane repair.

The EMBO journal·2026
Same author

Modular Photoswitchable Molecular Glues for Chemo-Optogenetic Control of Protein Function in Living Cells.

Angewandte Chemie (International ed. in English)·2025
Same author

A chemical inhibitor of IST1-CHMP1B interaction impairs endosomal recycling and induces noncanonical LC3 lipidation.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.

Nature communications·2023
Same author

ATG12-ATG5-TECPR1: an alternative E3-like complex utilized during the cellular response to lysosomal membrane damage.

Autophagy·2023
Same author

Inducin Triggers LC3-Lipidation and ESCRT-Mediated Lysosomal Membrane Repair.

Chembiochem : a European journal of chemical biology·2023

Related Experiment Video

Updated: Jun 2, 2025

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.1K

Visible-Light-Switchable Molecular Glues for Reversible Control of Protein Function.

Jun Zhang1,2, Laura K Herzog1,2, Shuang Li1,2

  • 1SciLifeLab, Department of Chemistry, Umeå University, 90187, Umeå, Sweden.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 13, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed new light-controlled molecular glues for reversible protein dimerization. These versatile chemo-optogenetic systems offer precise spatiotemporal control over biological processes in vitro and in cells.

Keywords:
CIDChemo-optogenetic systemMolecular gluePhotoswitchesVisible light

More Related Videos

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

10.4K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

489

Related Experiment Videos

Last Updated: Jun 2, 2025

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.1K
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

10.4K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

489

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Optogenetics

Background:

  • Chemically induced dimerization/proximity (CID/CIP) systems use molecular glues to control biological processes.
  • Existing systems often have limitations like irreversible photolysis.

Purpose of the Study:

  • To develop versatile chemo-optogenetic systems for reversible protein dimerization using light.
  • To overcome limitations of previous light-controlled dimerization systems.

Main Methods:

  • Utilized azobenzene-based photoswitchable molecular glues (sMGs).
  • Optimized photoswitch properties and linker strategies.
  • Applied visible light (blue, green, red) for control.

Main Results:

  • Achieved efficient and reversible protein dimerization using visible light.
  • Demonstrated multiple cycles of light-induced dimerization.
  • Showcased rapid and reversible control of protein function in vitro and in cells.

Conclusions:

  • Developed advanced chemo-optogenetic tools for precise spatiotemporal regulation.
  • These systems offer expanded applications in dynamic biological process manipulation.
  • Significant advancement in controlling biological systems with light.