Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy and safety of inhaled ambroxol hydrochloride solution in Chinese pediatric patients with acute lower respiratory tract infections: a real-world, multicenter, open-label, single-arm study.

Translational pediatrics·2026

Same author

Bio-recruiting hydrogel targeting mitochondrial homeostasis under neutrophil extracellular traps for diabetic wound repair.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

New insights into the ACLY-mediated metabolic and epigenetic interplay in macrophages.

Journal of enzyme inhibition and medicinal chemistry·2026

Same author

Nasal Aesthetic Restoration by Pre-expanded Paramedian Forehead Flap with Autologous Cartilage Framework and Laser Treatment.

Aesthetic plastic surgery·2026

Same author

Elucidating Tumorigenesis Mechanisms and Assessing Immunotherapeutic Efficacy in Patient-Derived Medulloblastoma Organoid Models.

International journal of biological sciences·2026

Same author

A Calcium Silicate-derived Silicate Ion Solution Improves Fat Graft Retention and Promotes Angiogenesis: An Experimental Study.

Aesthetic surgery journal·2026

Search research articles

Related Experiment Video

Updated: Jun 2, 2025

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

Published on: November 7, 2019

Constructing the Well Regenerated Decellularized Adipose Tissue Using External Volume Expansion Device.

Jie Long¹, Ping Xue¹, Yuge Zhang¹

¹Department of Plastic and Reconstruction Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.

Aesthetic Plastic Surgery

|January 13, 2025

Summary

External volume expansion (EVE) devices significantly enhance decellularized adipose tissue (DAT) regeneration by promoting stem cell recruitment and vascularization. This method improves adipose tissue formation and function.

Keywords:

Adipogenic microenvironment DAT EVE Inflammatory Tissue regeneration

More Related Videos

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

Related Experiment Videos

Last Updated: Jun 2, 2025

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

Published on: November 7, 2019

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

Area of Science:

Biomaterials science
Tissue engineering
Regenerative medicine

Background:

Decellularized adipose tissue (DAT) shows promise as a scaffold for adipose regeneration.
Current DAT effectiveness is limited, necessitating further investigation into regeneration mechanisms.

Purpose of the Study:

To explore external volume expansion (EVE) technology's potential to enhance DAT-mediated adipogenesis.
To investigate EVE's role in cellular recruitment and microenvironment modulation for adipose tissue regeneration.

Main Methods:

DAT was implanted in rats, with one group receiving daily EVE suction treatment for 14 days.
Tissue analysis included volume measurement, histology, immunofluorescence (CD34, CD90, CD68, CD31, perilipin), electron microscopy, and ELISA for cytokines (IL-1, TNFα, CCL2, CXCL12).

Main Results:

EVE treatment significantly increased DAT volume, porosity, and cellular adhesion.
Increased recruitment of adipose-derived stem cells (CD34/CD90) and macrophages (CD68) was observed with EVE.
EVE enhanced vascularization (CD31) and upregulated adipogenic factors (IL-1, TNFα, CCL2, CXCL12).

Conclusions:

External volume expansion (EVE) technology significantly improves decellularized adipose tissue (DAT) regeneration.
EVE promotes stem cell recruitment, macrophage activation, and adipogenesis-related cytokine expression, leading to enhanced functional adipose tissue formation.