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The Evolution of Nucleic Acid Nanotechnology: From DNA Assembly to DNA-Encoded Library.

Qigui Nie1,2, Xianfu Fang2,3, Jiale Huang2

  • 1Chongqing Fuling Hospital, Chongqing University, Chongqing, 40800, China.

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|January 14, 2025
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Summary

This review explores DNA nanotechnology and DNA-encoded libraries (DEL), highlighting their shared DNA assembly principles. These technologies offer versatile applications in biological sciences through innovative design and methodology.

Keywords:
DNA nanotechnologyDNA‐encoded libraryDNA‐templated reactiondynamic DNA nanotechnologyproximity inductionself‐assembly

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Materials Science

Background:

  • Deoxyribonucleic acid (DNA) is crucial for genetic information and has unique hybridization properties.
  • DNA's base pairing enables applications beyond biological functions, particularly in scientific research.
  • DNA assembly principles are fundamental to both DNA nanotechnology and DNA-encoded libraries.

Purpose of the Study:

  • To introduce and review DNA nanotechnology and DNA-encoded libraries (DEL).
  • To highlight the shared design principles of DNA assembly in both fields.
  • To explore potential synergies and future applications of combined technologies.

Main Methods:

  • Overview of structural and dynamic DNA nanotechnology approaches.
  • Examination of self-assembly-based DEL synthesis and DNA-templated reactions.
  • Analysis of common methodologies and design principles in DNA assembly.

Main Results:

  • DNA nanotechnology encompasses structural and dynamic approaches, including strand displacement and templated synthesis.
  • DEL synthesis utilizes DNA assembly for self-assembly, DTS, and proximity induction.
  • Shared principles in DNA assembly underpin advancements in both fields.

Conclusions:

  • DNA nanotechnology and DEL share fundamental design principles rooted in DNA assembly.
  • Synergies between these technologies can lead to novel functionalities and applications.
  • Further exploration of combined approaches promises versatile and sophisticated applications in biological sciences.