Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

92.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.7K
Sanger Sequencing01:57

Sanger Sequencing

757.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
757.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.5K
RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.6K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.6K
DNA Isolation01:24

DNA Isolation

40.7K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
40.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On-DNA Epoxide Synthesis and Ring-Opening Derivatization.

Organic letters·2026
Same author

DNA-Compatible Synthesis of β-Ketoamides as Intermediates for On-DNA Chemical Diversification.

Organic letters·2026
Same author

Accessing α-Ketoamides on DNA through a Coupling-Oxidation Strategy.

Organic letters·2026
Same author

Selenoxides unlock C-H bonds of biomacromolecules.

Nature chemistry·2025
Same author

DNA-Compatible Synthesis of Pyrrolidine-Fused Scaffolds via a One-Pot Three-Component Cycloaddition Strategy.

Organic letters·2025
Same author

Synthesis of Versatile DNA-Conjugated Aldehydes by Controlled Oxidation of Amines.

Angewandte Chemie (International ed. in English)·2025
Same journal

Aptamer-powered surveillance of SARS-CoV-3.

Chemical communications (Cambridge, England)·2026
Same journal

Does aurophilicity exist beyond the solid state?

Chemical communications (Cambridge, England)·2026
Same journal

Pressure-induced emission enhancement in 2-(anthracen-9-yl)-9<i>H</i>-thioxanthen-9-one crystals with π-π stacked thioxanthone dimers.

Chemical communications (Cambridge, England)·2026
Same journal

A Co-peptoid electrocatalyst for nitrite reduction that enables selective production of ammonia.

Chemical communications (Cambridge, England)·2026
Same journal

An AIE-based fluorescent probe for selective and sensitive detection of <i>N</i>-bromosuccinimide.

Chemical communications (Cambridge, England)·2026
Same journal

Harnessing the heteroatomic S/P coordination effects of FeCo dual-atomic catalysts for enhanced ORR performance.

Chemical communications (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Sep 17, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.3K

Recent advances in DNA-encoded libraries.

Yuting Gao1, Jinlu Liu1, Sijie Huang1

  • 1Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China. gongzhang@cqu.edu.cn.

Chemical Communications (Cambridge, England)
|July 1, 2025
PubMed
Summary
This summary is machine-generated.

DNA-encoded libraries (DELs) are revolutionizing drug discovery by combining chemistry and genetic barcoding. Recent advances in DEL technology over five years show significant progress in screening methods and library design for pharmaceutical research.

More Related Videos

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.4K

Related Experiment Videos

Last Updated: Sep 17, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.3K
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.4K

Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Drug Discovery

Background:

  • DNA-encoded libraries (DELs) integrate combinatorial chemistry and genetic barcoding for high-throughput screening.
  • DEL technology has evolved significantly over 30 years, becoming a key platform in pharmaceutical research.

Purpose of the Study:

  • To review recent advancements in DELs over the past five years.
  • To highlight progress in encoding, chemistry, selection, library design, and hit picking.
  • To identify addressed challenges and future research directions for DEL development.

Main Methods:

  • Review of recent literature and technological developments in DNA-encoded libraries.
  • Analysis of advancements in key DEL components: encoding, chemistry, selection, design, and hit picking.

Main Results:

  • Significant progress has been made in DEL encoding methods and compatible chemistries.
  • Enhanced selection techniques and library design strategies have improved hit identification.
  • Key challenges in DEL technology have been addressed, leading to breakthroughs.

Conclusions:

  • DELs represent a valuable and rapidly evolving technology for drug discovery.
  • Continued innovation in DELs promises further acceleration of pharmaceutical research and development.
  • Future directions focus on refining methods to maximize DELs' potential in identifying novel therapeutics.