Parameter Pool-Assisted Centrifugation Sorter for Multiscale Higher-Order DNA Nanomaterials
Lilin Ouyang1, Junke Wang1, Bing Liu1,2
1State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
ACS Nano
|January 15, 2025
Summary
A new centrifugation method precisely sorts complex DNA nanomaterials, overcoming limitations of traditional techniques. This advancement enables high-throughput characterization and enrichment of functional nanostructures for advanced applications.
Area of Science:
- Nanotechnology
- Biotechnology
- Materials Science
Background:
- Higher-order DNA nanomaterials offer programmability for biological studies and nanodevice construction.
- Hierarchical assembly of DNA nanomaterials often results in complex mixtures, hindering characterization and enrichment.
- Existing techniques like gel electrophoresis lack the resolution and scalability for complex DNA nanostructures.
Purpose of the Study:
- To develop a versatile, scalable sorting pipeline for precise characterization and enrichment of DNA nanomaterials.
- To overcome the limitations of current methods in resolving intricate mixtures of DNA nanostructures.
- To enable the application of DNA nanomaterials in complex functional material fabrication and molecular machine construction.
Main Methods:
- Development of a centrifugation parameter pool for distinguishing DNA nanomaterials across multiple scales.
- High-throughput classification of nanostructures from DNA tiles to DNA origami assemblies (∼50 MDa, 75,000 bp).
- Optimization of separation resolution to <78 kDa (∼120 bp) for DNA tetrahedron structures using systematic parameter pool-assisted centrifugation.
Main Results:
- Achieved high-throughput classification of DNA nanostructures, surpassing conventional methods in scale and resolution.
- Demonstrated optimized separation resolution of less than 78 kDa (∼120 bp) for DNA tetrahedron structures.
- Maintained the integrity and functionality of bioactive DNA nanomaterials during the sorting process.
Conclusions:
- The developed centrifugation sorting pipeline offers high resolution, broad scope, and bioactive functionality for DNA nanomaterials.
- This method facilitates seamless transition from assembly to application, enabling integration with other components.
- The technique supports the fabrication of complex functional materials and programmable molecular machines for interdisciplinary research.
