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Bacterial Isolates and Their Antimicrobial Susceptibility Patterns Among Pediatric Patients with Urinary Tract
Esmatullah Esmat1, Ramin Saadaat1, Noor Hassan Saedi1
1Department of Pathology and Clinical Laboratory, French Medical Institute for Mother and Children (FMIC), Kabul, Afghanistan.
Insights
Urinary tract infections (UTIs) are common in young children. This study identified common UTI-causing bacteria and their resistance patterns, highlighting the need for targeted antibiotic use to combat antimicrobial resistance.
Area of Science:
- Pediatric infectious diseases
- Microbiology
- Antimicrobial resistance
Background:
- Antibiotic resistance is a growing global health concern.
- Urinary tract infections (UTIs) are prevalent in pediatric populations.
- Nonspecific symptoms in young children can complicate UTI diagnosis.
Purpose of the Study:
- To identify common uropathogens in children under five with UTI symptoms.
- To determine the antimicrobial susceptibility spectrum of isolated organisms.
- To inform appropriate treatment strategies and combat antimicrobial resistance.
Main Methods:
- A descriptive cross-sectional study of 272 positive urine cultures from children under five.
- Analysis of isolated organisms and their antibiotic susceptibility.
- Data collected included patient demographics, urinalysis results, and culture findings.
Main Results:
- Escherichia coli, Klebsiella, Candida, and Enterococcus species were the most common isolates.
- 62% of gram-negative organisms were extended-spectrum beta-lactamase (ESBL) positive.
- High resistance rates were observed for Augmentin, Ampicillin, and third-generation cephalosporins, particularly in E. coli.
Conclusions:
- UTIs in pediatric patients require prompt diagnosis and appropriate management.
- Urine culture and sensitivity testing are crucial for guiding antibiotic therapy.
- Understanding local resistance patterns is essential for effective UTI treatment in children.
Introduction:
The widespread use of antibiotics is a serious and alarming situation in terms of the development of antimicrobial resistance. The current study was conducted to demonstrate the types of organism isolated from the urine of patients presenting with UTI symptoms as well as their antimicrobial sensitivity spectrum.
Methodology:
A descriptive cross-sectional study was conducted, and 272 positive urine cultures from children under 5 years of age with signs and symptoms of a UTI were included in the study. The types of organisms isolated from the urine cultures and their susceptibility to antibiotics were identified. The data collection form was designed as an Excel spreadsheet that included both dependent and independent variables, such as patient age, gender, WBC, red blood cell (RBC) count, nitrite, organism isolated, and antiprogram results.
Results:
Of the patients included, 64% were female. The majority were under one year of age, followed by children aged one to three. Among these children, 63% had pyuria and hematuria, and 64% had nitrite-positive urine samples. The most commonly isolated organisms included Escherichia coli, Klebsiella species, Candida species, Candida albicans, and Enterococcus species. In this study, 62% of gram-negative organisms were ESBL positive, among which the Proteus species demonstrated the highest ESBL positivity, followed by the Klebsiella species and E. coli. The majority of Enterobacteriaceae isolates in this study showed resistance to Augmentin and Ampicillin. Similarly, E. coli was highly resistant to third-generation cephalosporins, ceftazidime, and ceftriaxone.
Conclusion:
Due to the high prevalence of UTIs in pediatric patients and their nonspecific signs and symptoms, particularly in infants or young children, diagnosing and treating them, whilst difficult, is crucial. Urine samples should be analyzed for all pediatric patients with fever and, if pyuria is present, a urine culture is necessary.
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