Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nuclear dysfunction in aging and neurodegeneration.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Hearing Loss and Modifiable Dementia Risk Factors in Adults Aged 50 Years or Older Living in Tasmania, Australia.

Australasian journal on ageing·2026
Same author

Pre-operative proton versus photon-based chemoradiotherapy as an addition to best systemic therapy in the management of oesophageal cancer: protocol for the UK multi-centre randomised phase 2 PROTIEUS study.

BMC cancer·2026
Same author

Transcription Factor-Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors.

bioRxiv : the preprint server for biology·2026
Same author

Increased synaptic turnover in injured cortical axons: exploring the role of SARM1 ablation.

Frontiers in synaptic neuroscience·2026
Same author

Association of chronic pain and analgesic use with the risks of Parkinson's and Alzheimer's disease.

GeroScience·2025

Related Experiment Video

Updated: Jun 2, 2025

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.6K

Combining Top-Down and Bottom-Up: An Open Microfluidic Microtumor Model for Investigating Tumor Cell-ECM Interaction

Chao Li1,2, Jiayi Li3, Zach Argall-Knapp4

  • 1Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, 53792, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|January 15, 2025
PubMed
Summary

A novel Under-oil Open Microfluidic System (UOMS) enables precise study of tumor cell migration and metastasis. This microfluidic model effectively tested an anti-metastasis drug, showing promising results in suppressing tumor cell movement and extracellular matrix remodeling.

Keywords:
ECM alignment&remodelinganti‐metastasiscell migrationopen microfluidicstumor microenvironment

More Related Videos

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.5K
Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

1.6K

Related Experiment Videos

Last Updated: Jun 2, 2025

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.6K
Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.5K
Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

1.6K

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Microfluidics

Background:

  • Investigating tumor cell migration and metastasis is crucial for developing effective anti-cancer therapies.
  • Current microfluidic models often lack the ability to replicate the complex tumor microenvironment and allow for dynamic manipulation.
  • There is a need for advanced in vitro models that can accurately predict drug efficacy against cancer metastasis.

Purpose of the Study:

  • To develop and validate an Under-oil Open Microfluidic System (UOMS)-based microtumor model for studying tumor cell migration and anti-metastasis drug screening.
  • To compare the UOMS model's capabilities with traditional closed microfluidic systems.
  • To assess the efficacy of an anti-metastasis drug (incyclinide, CMT-3) on triple-negative breast cancer cells (MDA-MB-231) within the UOMS model.

Main Methods:

  • Utilized a combined top-down (operator-directed) and bottom-up (cell-directed) strategy for microtumor model creation.
  • Employed an open microfluidic design with micrometer-scale surface patterning for spatiotemporal sample manipulation.
  • Incorporated self-organized extracellular matrix (ECM) structures and spontaneous tumor cell-ECM remodeling within the UOMS.
  • Tested the anti-metastasis drug incyclinide (CMT-3) on MDA-MB-231 cells in the UOMS model.

Main Results:

  • The UOMS model demonstrated micrometer-scale lateral resolution and flexible sample manipulation.
  • Observed self-organized ECM structures and spontaneous tumor cell-ECM remodeling, mimicking in vivo conditions.
  • The UOMS model allowed for controlled yet self-organized tumor-ECM microenvironment in an open configuration.
  • In vitro testing showed that incyclinide (CMT-3) suppressed tumor cell migration and ECM remodeling.

Conclusions:

  • The UOMS-based microtumor model provides a versatile platform for investigating tumor cell migration and metastasis.
  • The model successfully replicated key aspects of the tumor microenvironment, including ECM remodeling.
  • In vitro results using the UOMS model correlated with in vivo metastasis data, validating its predictive potential for anti-metastasis drug efficacy.