Clinical Microbiome Analysis by Mass Spectrometry-Based Metaproteomics

Area of Science:

• Microbiology
• Proteomics
• Bioinformatics

Background:

• Mass spectrometry-based proteomics and metaproteomics are established tools for human microbiome research.
• Recent technological advancements have unlocked the full potential of metaproteomics.
• Progress is driven by high-performance mass spectrometers, innovative proteomics strategies, and bioinformatics tools.

Purpose of the Study:

• To critically examine recent technological developments enhancing metaproteomics in clinical microbiome analysis.
• To summarize advancements in applying metaproteomics to human microbiomes in disease states.
• To highlight key aspects for broader and more effective clinical application of metaproteomics.

Main Methods:

• Review of recent technological developments in mass spectrometry and proteomics.
• Analysis of advancements in bioinformatics tools for metaproteomics.
• Synthesis of findings from studies on human microbiomes across various body sites and disease conditions.

Main Results:

• Technological progress has significantly enhanced metaproteomics capabilities for microbiome studies.
• Metaproteomics has shown significant advancements in understanding human microbiomes in various diseases.
• Underutilization persists due to small sample sizes and insufficient data mining.

Conclusions:

• Metaproteomics holds vast potential for clinical microbiome analysis.
• Further development of microbiome assays for translational research is crucial.
• Addressing limitations in sample size and data mining will facilitate wider application.