Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Measuring the Acoustic Reflex through the Tympanic Membrane.

Audiology & neuro-otology·2024

Same author

Pinhole shifting for reducing speckle contrast in reflectance confocal microscopy.

Optics letters·2023

Same author

In vivo optical mapping of the tympanic membrane impulse response.

Hearing research·2023

Same author

Measuring the red blood cell shape in capillary flow using spectrally encoded flow cytometry.

Biomedical optics express·2022

Same author

Optimization study of plasmonic cell fusion.

Scientific reports·2022

Same author

Plasmonic fusion between fibroblasts and skeletal muscle cells for skeletal muscle regeneration.

Biomedical optics express·2022

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 2, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Spectrally encoded flow cytometry using few-mode fiber collection.

Reut Friedman¹, Dvir Yelin¹

¹Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.

Biomedical Optics Express

|January 16, 2025

Summary

This summary is machine-generated.

This study demonstrates a novel method to eliminate spectral modulation artifacts in fiber-based confocal microscopy. Mode-dependent polarization control improves spectrally encoded imaging, enhancing signal efficiency and reducing noise.

More Related Videos

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 2, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

Area of Science:

Optics and Photonics
Microscopy Techniques
Fiber Optics

Background:

Fiber-based confocal microscopy utilizes separate illumination and collection fibers for pinhole-like effects.
Multimode fiber propagation in collection can cause differential mode delay, leading to spectral modulation artifacts in coherent detection.
These artifacts are problematic for Fourier-domain and spectral measurement applications.

Purpose of the Study:

To eliminate spectral modulation artifacts in fiber-based confocal microscopy.
To improve spectrally encoded imaging performance.
To enhance signal efficiency and reduce speckle noise.

Main Methods:

Utilized a few-mode fiber for illumination and collection.
Implemented mode-dependent polarization control to mitigate differential mode delay.
Demonstrated spectrally encoded imaging with the developed technique.

Main Results:

Successfully eliminated spectral modulation artifacts.
Achieved improved signal efficiency in spectrally encoded imaging.
Observed a reduction in speckle noise.
Reported a minor, negligible decrease in lateral and axial resolution.

Conclusions:

Mode-dependent polarization control is effective in removing spectral artifacts in fiber-based confocal microscopy.
The developed method enhances spectrally encoded imaging with improved signal efficiency and reduced noise.
This technique offers a viable solution for applications requiring spectral measurements with fiber-based systems.