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Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control.

Hyun Pyo Kim1, Ji-Ho Park2, Min-Gon Kim3

  • 1AI the nutrigene, 42, Changeop-Ro, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do, 13449, Republic of Korea.

Mikrochimica Acta
|January 17, 2025
PubMed
Summary

A new lab-on-paper platform offers affordable, rapid molecular diagnostics for field testing. This cost-effective system enables sensitive detection of foodborne pathogens within 30 minutes, ideal for point-of-care applications.

Keywords:
Disposable testFood poisoningIsothermal amplificationLab-on-paperPoint-of-careVisual detection

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Growing demand for accessible, low-cost molecular diagnostics in global healthcare.
  • Need for rapid, field-deployable testing solutions, especially in resource-limited settings.

Purpose of the Study:

  • Introduce and evaluate an innovative lab-on-paper (LOP) platform for molecular diagnostics.
  • Demonstrate the platform's capability for rapid, sensitive, and cost-effective detection of microorganisms.

Main Methods:

  • Development of an automated microfluidics-based LOP system integrating isothermal amplification and paper strip detection.
  • Utilized USB-powered thermal management and wax valve mechanisms for process control.
  • Colorimetric detection assay for foodborne pathogens.

Main Results:

  • Successful detection of Vibrio vulnificus (120 CFU/mL) and Salmonella Typhimurium (60 CFU/mL) with naked-eye visualization.
  • Complete assay time (amplification and detection) achieved within 30 minutes.
  • Demonstrated assay cost of $5.2 USD per test, highlighting cost-effectiveness.

Conclusions:

  • The LOP platform provides a rapid, sensitive, and affordable solution for molecular diagnostics.
  • Its portability and ease of use make it suitable for point-of-care testing in diverse environments.
  • The technology holds significant potential for infectious disease monitoring and food safety applications.