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Published on: January 21, 2020
A Modified Ligature-Induced Peri-Implantitis Murine Model and RNA Sequencing Analysis Compared With Human Subjects
Zi-An Tong1, Long Chen1, Ling Shen1
1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, Zhejiang, China.
A modified mouse model effectively mimics human peri-implantitis, showing similar gingival gene expression and immune cell infiltration, particularly macrophages and neutrophils, aiding research into this condition.
Area of Science:
- Oral biology
- Immunology
- Genomics
Background:
- Peri-implantitis is a significant complication of dental implants.
- Understanding its pathogenesis requires suitable preclinical models.
- Current models may not fully replicate human disease characteristics.
Purpose of the Study:
- To develop and validate a modified ligature-induced peri-implantitis murine model.
- To analyze gene expression and immune cell infiltration in peri-implant tissues.
- To compare transcriptomic profiles between the murine model and human peri-implantitis patients.
Main Methods:
- Established a ligature-induced peri-implantitis model in mice via immediate implant placement.
- Utilized RNA sequencing for transcriptomic profiling of mouse and human peri-implant tissues.
- Conducted histopathological and bioinformatics analyses for immune cell infiltration and inflammation assessment.
Main Results:
- Mouse gingival tissues exhibited robust immune and inflammatory responses, with notable macrophage activity.
- Alveolar bone in mice showed comparatively weaker immune and inflammatory reactions.
- Transcriptomic analysis revealed similar gene expression patterns and increased macrophage and neutrophil infiltration in the gingiva of both humans and mice.
Conclusions:
- The modified murine model demonstrates significant transcriptomic similarities to human peri-implantitis, particularly in gingival tissues.
- Increased immune functions and inflammation were observed in mouse gingival tissue during peri-implantitis progression.
- Macrophages and neutrophils are identified as potentially critical immune cells in peri-implantitis pathogenesis, supporting the model's utility for further research.
