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Updated: Jun 1, 2025

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
Published on: February 11, 2020
Development and validation of headspace gas chromatography with a flame ionization detector method for the
Filip Mihajlović1, Ivana Andrić2, Živana Slović2,3
1Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, 34000, Serbia.
Introduction:
Qualitative and quantitative testing of ethanol in post-mortem samples is an important analytical procedure that provides accurate, precise, and reliable results. Given the complexity of the issue, obtaining a realistic picture of lifelong alcoholemia requires supporting blood ethanol findings with analyses of alternative samples, primarily vitreous humor (VH).
Objective:
The objective of this study was to develop and validate a headspace gas chromatography with flame ionization detection (HS/GC-FID) method for determining ethanol concentration in VH.
Materials And Methods:
Conditions for the HS/GC-FID method were established and the method was validated according to the guidelines of the European Medicines Agency. Validation parameters such as precision, accuracy, specificity, sensitivity, and linearity over a wide concentration range were evaluated through statistical analysis.
Results:
The method demonstrated precision, accuracy, specificity, and sensitivity. Additionally, it proved to be linear across a wide concentration range and relatively fast, making it suitable for rapid and routine determination of ethanol concentration in VH, particularly for forensic applications.
Conclusion:
Results from validation and application of the method to VH samples indicate that ethanol concentration in VH can be reliably determined using the presented HS/GC-FID method, making it a valuable tool in forensic investigations.
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