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Generation of induced pluripotent stem cell line from a patient with long COVID

David Wu1, Amit Manhas2, Chikage Noishiki1

  • 1Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA.

Stem Cell Research
|January 17, 2025
PubMed

Insights

Long COVID causes vascular dysfunction and increased clotting risk. Researchers created a stem cell line from a patient to study these long-term effects and multi-organ damage.

Area of Science:

  • Stem cell biology
  • Infectious disease research
  • Vascular medicine

Background:

  • Long COVID, or post-acute sequelae of SARS-CoV-2 infection, is linked to vascular dysfunction and multi-organ damage.
  • COVID-19-associated coagulopathy (CAC) signifies an increased thrombotic risk in Long COVID patients.
  • Understanding the mechanisms of Long COVID is crucial for developing effective treatments.

Purpose of the Study:

  • To derive and characterize a human induced pluripotent stem cell (iPSC) line from a Long COVID patient.
  • To establish a cellular model for investigating the pathogenesis of Long COVID.
  • To facilitate research into the vascular and multi-organ complications associated with SARS-CoV-2 infection.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were collected from a Long COVID patient.
  • PBMCs were reprogrammed into induced pluripotent stem cells (iPSCs).
  • The derived iPSC line was characterized for morphology, pluripotency, karyotype stability, and differentiation potential.

Main Results:

  • A stable iPSC line was successfully derived from a Long COVID patient.
  • The iPSC line exhibited normal morphology and maintained pluripotency.
  • The iPSC line demonstrated successful differentiation into ectoderm, endoderm, and mesoderm germ layers.

Conclusions:

  • The established iPSC line serves as a valuable tool for modeling Long COVID.
  • This cellular model can aid in exploring the mechanisms of vascular dysfunction and multi-organ damage in Long COVID.
  • Further research using this iPSC line may uncover novel therapeutic strategies for Long COVID complications.

Keywords:
Induced pluripotent stem cellsInflammationLong COVID

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