Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genetics and outcomes of an altered FGF23-1,25D-PTH axis in diseases of mineral metabolism.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same author

The Kidney Precision Medicine Project and Single-Cell Biology of the Injured Proximal Tubule.

The American journal of pathology·2024
Same author

miRNA and mRNA Signatures in Human Acute Kidney Injury Tissue.

The American journal of pathology·2024
Same author

Dynamic Single Cell Transcriptomics Defines Kidney FGF23/KL Bioactivity and Novel Segment-Specific Inflammatory Targets.

bioRxiv : the preprint server for biology·2024
Same author

miRNA and mRNA Signatures in Human Acute Kidney Injury Tissue.

bioRxiv : the preprint server for biology·2023
Same author

Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23.

Bone research·2023

Related Experiment Video

Updated: Jul 16, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

14.9K

Dynamic single cell transcriptomics defines kidney FGF23/KL bioactivity and novel segment-specific inflammatory

Rafiou Agoro1, Jered Myslinski2, Yamil G Marambio3

  • 1Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana, USA; The Jackson Laboratory, Bar Harbor, Maine, USA.

Kidney International
|January 19, 2025
PubMed
Summary

Fibroblast growth factor 23 (FGF23) signaling in the kidney is detailed at single-cell resolution. FGF23 actions depend on αKlotho (KL) and influence phosphate metabolism via MAPK and vitamin D pathways.

Keywords:
FGF23distal tubuleinflammationmineral metabolismproximal tubule

More Related Videos

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

4.4K
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
10:25

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

1.7K

Related Experiment Videos

Last Updated: Jul 16, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

14.9K
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

4.4K
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
10:25

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

1.7K

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genomics

Background:

  • Fibroblast growth factor 23 (FGF23) and its coreceptor αKlotho (KL) are crucial for phosphate metabolism.
  • Dysregulation of FGF23/KL signaling is implicated in various kidney diseases.
  • The precise spatial-temporal mechanisms of FGF23 action in the kidney are not fully understood.

Purpose of the Study:

  • To elucidate the single-cell resolution mechanisms of kidney FGF23 bioactivity.
  • To identify specific cell types and pathways regulated by FGF23 in vivo.
  • To uncover novel FGF23-dependent signaling crosstalk.

Main Methods:

  • Single-cell RNA sequencing (scRNAseq) of mouse kidneys after FGF23 injection.
  • Computational analysis of cell clusters and differential gene expression.
  • Integration of ATACseq and RNAseq data from a KL-expressing cell line.
  • In vitro and in vivo validation of identified pathways.

Main Results:

  • FGF23 bioactivity was mapped to specific epithelial, endothelial, stromal, and immune cell populations.
  • KL expression in proximal tubules and distal convoluted tubules is critical for FGF23 action.
  • Temporal FGF23 responses modulated MAPK signaling and vitamin D metabolism via distinct transcriptional regulons.
  • Unexpected crosstalk between FGF23-MAPK and TNF receptor/NF-κB signaling was identified, inhibiting FGF23 bioactivity.

Conclusions:

  • Novel FGF23-dependent pathways were uncovered at the single-cell level in the kidney.
  • These findings provide insights into FGF23-related kidney diseases.
  • Understanding these mechanisms may aid in developing targeted therapeutic strategies.