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Label-Free 3D Cell Imaging Using Hydrogels Functionalized with Switchable Iridium Complexes.

Federica Fiorini1,2, Elena Longhi1,3, Ariadna Lazaro4

  • 1Institut de Science et d'Ingénierie Supramoléculaires (I.S.I.S.), Université de Strasbourg, 8 allée Gas-pard Monge, Strasbourg, 67000, France.

Chemistry (Weinheim an Der Bergstrasse, Germany)
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Summary

This study introduces a novel hydrogel scaffold for cell visualization. The scaffold uses luminescent iridium complexes that activate upon cell membrane interaction, enabling real-time, staining-free 3D cell tracking.

Keywords:
3D hydrogelsiridium complexreal-time cells visualizationstaining-less“off-on” luminescence

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Nanotechnology

Background:

  • Traditional fluorescent labels can alter cell behavior.
  • Need for non-invasive cell visualization techniques.

Purpose of the Study:

  • Develop a staining-free method for real-time 3D cell visualization.
  • Utilize luminescent iridium complexes for cell tracking.

Main Methods:

  • Functionalizing a 3D hydrogel scaffold with cyclometalated iridium(III) complexes.
  • Designing iridium complexes that are emissive only upon interaction with cell membranes.
  • Exploiting luminescence quenching in aqueous environments.

Main Results:

  • Iridium-functionalized hydrogels exhibit weak luminescence in culture media.
  • Cell interaction with the hydrogel "switches on" luminescence.
  • Achieved clear, dynamic, real-time 3D visualization of cell proliferation.

Conclusions:

  • The developed iridium-functionalized hydrogel enables staining-free, real-time 3D cell tracking.
  • This methodology offers a promising approach for monitoring dynamic cellular processes.
  • Potential applications in cell proliferation studies and advanced imaging.