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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Chromatin Modification in iPS Cells01:32

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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Updated: Jun 1, 2025

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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DESIGNER FAT CELLS: ADIPOGENIC DIFFERENTIATION OF CRISPR-CAS9 GENOME-ENGINEERED INDUCED PLURIPOTENT STEM CELLS.

E V Ely1,2,3,4, A T Kapinski1,2,3,4, S G Paradi1,2,3

  • 1Department of Orthopedic Surgery, Washington University in Saint Louis, Saint Louis, MO 63110, USA.

European Cells & Materials
|January 20, 2025
PubMed
Summary

Researchers developed a novel method to create engineered fat cells from stem cells. These designer fat cells can be genetically modified for studying diseases and developing new therapies.

Keywords:
Obesityadipocyte differentiationadipokine secretionadipose tissuecell-based therapiesfunctional adipocytestissue engineering

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Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Endocrinology

Background:

  • Adipose tissue functions as an endocrine organ, influencing systemic health.
  • Obesity-associated adipose tissue inflammation contributes to comorbidities.
  • Genetically engineered adipose tissue holds potential for therapeutic applications and disease modeling.

Purpose of the Study:

  • To develop a non-viral method for differentiating genome-edited murine induced pluripotent stem cells (iPSCs) into adipocytes.
  • To assess the in vivo engraftment potential of these designer adipocytes.
  • To establish a platform for studying adipose tissue signaling and interorgan communication.

Main Methods:

  • Utilized CRISPR-Cas9 for genetic engineering of iPSCs.
  • Induced adipogenic differentiation of iPSCs.
  • Transplanted designer adipocytes into lipodystrophic mice.
  • Developed methods for inducing adipocyte browning and whitening.

Main Results:

  • Successfully created designer adipocytes from genome-edited iPSCs.
  • Demonstrated the ability of these cells to undergo adipogenic differentiation.
  • Established a model for studying engineered adipose tissue in vivo.
  • Showcased potential for therapeutic applications and disease modeling.

Conclusions:

  • A novel platform for generating and studying engineered adipose tissue was established.
  • This approach facilitates research into adipokine signaling and interorgan communication.
  • The developed technology offers innovative strategies for regenerative medicine and disease treatment.