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Confirming Subarachnoid Hemorrhage Induction in the Endovascular Puncture Mouse Model
Ari Dienel1, Sung-Ha Hong1, Jose Guzman1
1The Vivian L. Smith Department of Neurosurgery, The University of Texas Health Science Center at Houston, Houston, Texas, USA.
Brain Hemorrhages
|January 20, 2025
Summary
Intracranial pressure (ICP) monitoring is the best method to confirm subarachnoid hemorrhage (SAH) induction in mice. Contralateral cerebral blood flow (CBF) monitoring can also detect SAH, but ipsilateral CBF is unreliable.
Area of Science:
- Neuroscience
- Cerebrovascular Research
- Animal Models
Background:
- Subarachnoid hemorrhage (SAH) research models require accurate induction confirmation.
- Cerebral blood flow (CBF) and intracranial pressure (ICP) are commonly used monitoring methods.
Purpose of the Study:
- To evaluate if contralateral CBF is a superior marker for SAH induction compared to ipsilateral CBF.
- To compare the reliability of CBF and ICP monitoring in an endovascular perforation SAH model.
Main Methods:
- Sixty-eight mice underwent endovascular perforation to induce SAH.
- Relative CBF was monitored using laser Doppler, and ICP was measured with probes.
- Simultaneous monitoring of ipsilateral and contralateral CBF was performed.
Main Results:
- Ipsilateral CBF decreased before ICP elevation.
- Contralateral CBF reduction occurred concurrently with ICP rise.
- Ipsilateral CBF drop preceded contralateral CBF drop when both were monitored.
Conclusions:
- Intracranial pressure (ICP) monitoring is the most suitable method for confirming SAH induction in this model.
- Contralateral CBF monitoring can detect SAH induction.
- Ipsilateral CBF monitoring is unsatisfactory due to occlusion artifacts.
