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Related Experiment Video

Updated: Jun 1, 2025

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Quantifying Pulmonary Microvascular Density in Mice Across Lobules.

Chunyan Li1, Zhu Wang2, Junrong Du1

  • 1Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University.

Journal of Visualized Experiments : Jove
|January 20, 2025
PubMed
Summary

This study introduces a new method to accurately measure pulmonary microvascular density in mice, accounting for lung lobe variations. This approach reveals age-related changes in lung microvasculature, aiding research into lung diseases.

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Area of Science:

  • Pulmonary research
  • Vascular biology
  • Respiratory system science

Background:

  • Abnormal pulmonary angiogenesis contributes to lung microvascular dysfunction, impacting vascular integrity, blood flow, and gas exchange.
  • Existing methods for quantifying lung microvascular density overlook significant variations in murine lung lobe size, shape, and vascularization.
  • This oversight limits comprehensive studies on lung microvascular dysfunction and lobule-specific microvasculature remodeling.

Purpose of the Study:

  • To develop and validate a novel protocol for unbiased quantification of pulmonary microvascular density across different lung lobes in mice.
  • To investigate age-related changes in microvascular density within distinct lung lobules.
  • To provide a cost-effective and straightforward method for assessing lung microvasculature in physiological and pathological contexts.

Main Methods:

  • Utilized two distinct sectioning techniques to analyze pulmonary microvascular density changes.
  • Leveraged airway branch morphology (size, shape, distribution) to differentiate and analyze distinct lung lobes.
  • Employed Isolectin B4 (IB4) staining for endothelial cell labeling and ImageJ software for unbiased microvascular density analysis.

Main Results:

  • Demonstrated significant variations in microvascular density across different lung lobules.
  • Observed distinct patterns of microvascular density changes associated with aging in mice.
  • Validated the protocol's effectiveness in providing unbiased quantification of microvascular density.

Conclusions:

  • The developed protocol accurately quantifies pulmonary microvascular density, considering inter-lobar variations in mice.
  • Aging is associated with lobule-specific alterations in lung microvascular density.
  • This method offers a valuable tool for advancing research into lung physiology and pathology.