Related Experiment Video
Updated: Jun 1, 2025
Author Spotlight: Rabies-Specific Antibody Isotypes Detection in Sera or Cerebral Spinal Fluid Using an IFA Test
Published on: January 19, 2024
Comparison Between Indirect Fluorescent Antibody Test and Modified Agglutination Test for Detecting Anti-Toxoplasma
Nycolas Octavio Ribeiro Carvalho1, Raquel Cuba Gaspar1, Suellen Gonçalves Lima1
1School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
Background:
Toxoplasma gondii is a zoonotic parasite with a great impact on the health of Neotropical primates (NP). The aim of this study was to compare the agreement between the immunofluorescent antibody test (IFAT) and the modified agglutination test (MAT) to detect IgG anti-T. gondii antibodies in NP.
Methods:
Convenience serum samples of 77 animals were tested by IFAT and MAT according to standard protocols, and the agreement between results was analyzed using McNemar's χ2 test.
Results:
The samples included 30 red-handed howlers (Alouatta belzebul), 24 brown howler monkeys (Alouatta guariba), 22 capuchin monkeys (Sapajus nigritus), and one wedge-capped capuchin (Cebus olivaceous). Overall prevalence was 25.9% (n = 20), with three animals positive by IFAT and 20 positives by MAT. The agreement between tests was low (Kappa = 0.21).
Conclusion:
The results indicated that IFAT and MAT are not comparable to assess qualitatively anti-T. gondii IgG serum antibodies in the studied species.
More Related Videos
06:333-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
Published on: December 9, 2014
11:21Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
