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On-Chip Stimulated Raman Scattering Imaging and Quantification of Molecular Diffusion in Aqueous Microfluidics.

Simin Bi1, Yumo Li1, Jianpeng Ao1

  • 1State Key Laboratory of Surface Physics and Department of Physics, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Shanghai Key Laboratory of Metasurfaces for Light Manipulation, Fudan University, Shanghai 200433, China.

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|January 22, 2025
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Summary

This study introduces a novel optical imaging technique combining stimulated Raman scattering (SRS) microscopy and microfluidics to rapidly measure molecular diffusion coefficients. The method offers high resolution and minimal sample volume, advancing the study of chemical reactions and biological processes.

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Area of Science:

  • Chemical Physics
  • Biophysics
  • Analytical Chemistry

Background:

  • Molecular diffusion is crucial in aqueous solutions for chemical reactions and life processes.
  • Conventional diffusion measurement methods are often complex, require large samples, and are time-consuming.

Purpose of the Study:

  • To develop a rapid, high-resolution optical imaging method for measuring molecular diffusion coefficients.
  • To overcome the limitations of conventional diffusion measurement techniques.

Main Methods:

  • Utilized stimulated Raman scattering (SRS) microscopy integrated with microfluidics.
  • Employed a "Y"-shaped microfluidic channel to create stable laminar flows and concentration gradients.
  • Applied the convection-diffusion model to extract diffusion coefficients from SRS imaging data.

Main Results:

  • Successfully measured diffusion coefficients for water, protein, and various ions.
  • Achieved measurements with sample volumes under 1 mL and time costs under 10 minutes.
  • Demonstrated high-resolution three-dimensional (3D) reconstruction of diffusion patterns.

Conclusions:

  • The microfluidic SRS platform provides a fast and efficient method for quantitative molecular diffusion measurements.
  • This technique has potential applications in studying chemical reactions, fluid dynamics, and liquid-liquid interfaces.