Pyro-Thermolysis Pattern Analysis of Selenopeptide in Selenium-Enriched Rice Based on Two-Dimensional Dietary...

Selenopeptides can be ideal dietary selenium (Se) supplements for humans. Currently, rice is not used much as a source of selenopeptides. Here, we executed the selenopeptidomics analysis of selenium-enriched rice protein hydrolysates using the full MS-dd-MS2 acquisition method and identified selenopeptides, including L{Se-Met}AK and other selenopeptides. Specifically, selenomethionine (SeMet) replaced methionine (Met) in the rice protein-Oryzain alpha chain (EC: 3.4.22) and generated a selenopeptide L{Se-Met}AK (molecular formula: C₂₀H₃₈N₅O₅Se) during subsequent protein hydrolysis. This selenopeptide was in 425-428 amino acid residues of the Oryzain alpha chain. Thermal processing led to selenopeptide cleavage, which affected the efficient retention of selenopeptides. Activation energy (E_a) was used to locate the quality control markers in the thermal degradation of selenopeptides. Therefore, this study established the thermal degradation rate equation for the selenopeptide L{Se-Met}AK at 100 °C, 110 and 120 °C; and identified the pyrolysis products, including L{Se-Met}A, LMA, LMAK, K₁, and K₂, involving C-N cleavage on the amide bond of alanine and lysine, C-Se bond cleavage and C-N cleavage on the amide bond of alanine and Met; the fit coefficients of the thermal reaction models were ≥0.9248, which could accurately quantify the real-time pyrolysis kinetic process; and LMAK had a lower E_a of 88.20 kJ/mol, which made it easier to produce. In summary, LMAK can be used as a quality control marker in the pyrolysis process, providing technical support for the efficient retention of selenopeptides.