Cellulose nanofibers/polyacrylic acid hydrogels integrated with a 3D printed strip: A platform for screening prostate cancer via sarcosine detection

Cellulose nanofiber/polyacrylic acid (CNF/PAA) hydrogel-based colorimetric sensor was fabricated for non-invasive screening of prostate cancer (PCa) via selective detection of sarcosine. The hydrogel was synthesized by photo-crosslinking of acrylic acid in the presence of CNF which acted as mechanical reinforcement and as color enhancer. The hydrogel exhibited a high aqueous absorption and high mechanical strength. A homogeneous distribution of CNF in the hydrogel was confirmed by TEM. A significant improvement in the compressive modulus and stress in the hydrogel were obtained after the incorporation of 0.25%wt CNF. The hydrogel sensor was integrated within a 3D printing strip on a diaper, and it offered a vivid color change from light yellow to blue for detecting sarcosine for PCa indication with a detection limit starting from 10 μM. The colorimetric results were semi-quantitatively evaluated by a spectrophotometer offering a linear range of 0-100 μM with R² of 0.9901. Furthermore, the increase in CNF content significantly enhanced the sensor's sensitivity toward sarcosine. This sensor could open new avenues for non-invasive screening of prostate cancer in the future.