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Updated: May 31, 2025

Electron Cryotomography of Bacterial Cells
Published on: May 6, 2010
Imaging of dairy emulsions via a novel approach of transmission electron cryogenic microscopy using beam exposure
Kilian Daffner1, Eric Hanssen2,3, Ian Norton1
1Department of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Abstract:
Emulsions containing small fat and protein particles are difficult to differentiate but low dose transmission electron cryogenic microscopy can visualize and distinguish between these droplets or particles. A controlled increase in electron dose caused the sample to bubble and visibly degrade at <25 e- Å-2 for fat globules and at >150 e- Å-2 for protein particles, allowing particle differentiation. This technique may be useful for the development of nanoemulsions, as well as nanostructured and 3D printed foods.
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