Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.3K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlation of Processing and Structure in an Ethylene-Glycol Side-Chain Modified Polythiophene via Combined X-Ray Scattering and 4D Scanning Transmission Electron Microscopy.

Small methods·2024
Same author

Mapping Polar Distortions using Nanobeam Electron Diffraction and a Cepstral Approach.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2023
Same author

Steam-created grain boundaries for methane C-H activation in palladium catalysts.

Science (New York, N.Y.)·2021
Same author

NexusLIMS: A Laboratory Information Management System for Shared-Use Electron Microscopy Facilities.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2021
Same author

Cold Microscopy Solves Hot Problems in Energy Research.

Matter·2020
Same author

Influence of Polymer Aggregation and Liquid Immiscibility on Morphology Tuning by Varying Composition in PffBT4T-2DT/Non-Fullerene Organic Solar Cells.

Advanced energy materials·2020
Same journal

Immunofluorescence study and morphometric analysis of collagen-IV in the exchange tissue of the quail (Coturnix coturnix) lung by confocal laser scanning microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

Micromorphological study of leaf surfaces structures in selected Crataegus L. (Rosaceae) species using light, scanning electron, and confocal laser microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

3D reconstruction of the nymphal feeding apparatus of Philaenus spumarius.

Micron (Oxford, England : 1993)·2026
Same journal

The influence of physicians and surgeons on Leeuwenhoek's observations of crystal formation.

Micron (Oxford, England : 1993)·2026
Same journal

Distribution of telocytes in the choroid (eye) of a teleost: An ultrastructural observation.

Micron (Oxford, England : 1993)·2026
Same journal

SEM-EDAX: A tool for microanalytical elemental mapping in butterfly wing scales.

Micron (Oxford, England : 1993)·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

10.3K

ETSpy: A HyperSpy extension package for electron tomography data processing and reconstruction.

Andrew A Herzing1, Joshua A Taillon2

  • 1Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.

Micron (Oxford, England : 1993)
|January 23, 2025
PubMed
Summary
This summary is machine-generated.

ETSpy is a new open-source software package for electron tomography. It simplifies pre-processing steps like spatial binning and alignment for nano- and atomic-scale material characterization.

Keywords:
Electron tomographyHyperSpyOpen sourceSoftware

More Related Videos

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.3K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

6.3K

Related Experiment Videos

Last Updated: May 7, 2026

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

10.3K
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.3K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

6.3K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Microscopy

Background:

  • Electron tomography is crucial for 3D material characterization at nano- and atomic scales.
  • Conventional tomography workflows involve complex pre-processing steps.
  • Existing software may not offer comprehensive solutions for these pre-processing challenges.

Purpose of the Study:

  • Introduce ETSpy, a new open-source software package for electron tomography.
  • Demonstrate the capabilities and ease of use of ETSpy.
  • Showcase specific applications of ETSpy in material characterization.

Main Methods:

  • Developed ETSpy as an open-source package, building upon the HyperSpy framework.
  • Implemented functionalities for essential pre-processing steps in electron tomography.
  • Presented basic usage scenarios and specific application examples.

Main Results:

  • ETSpy offers a streamlined approach to electron tomography pre-processing.
  • The software effectively handles tasks like spatial binning, image registration, and tilt-axis alignment.
  • Demonstrated successful application of ETSpy in analyzing nano- and atomic-scale materials.

Conclusions:

  • ETSpy provides a valuable, user-friendly tool for researchers in electron tomography.
  • The open-source nature of ETSpy promotes accessibility and further development.
  • ETSpy enhances the efficiency and effectiveness of 3D material characterization using electron tomography.