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Updated: May 31, 2025
A Web Tool for Generating High Quality Machine-readable Biological Pathways
Published on: February 8, 2017
BAC-browser: the tool for synthetic biology
Tatiana A Semashko1,2, Gleb Y Fisunov3,4, Georgiy Y Shevelev5
1Research Institute for Systems Biology and Medicine, Moscow, Russian Federation. t.semashko@gmail.com.
Background:
Currently, synthetic genomics is a rapidly developing field. Its main tasks, such as the design of synthetic sequences and the assembly of DNA sequences from synthetic oligonucleotides, require specialized software. In this article, we present a program with a graphical interface that allows non-bioinformatics to perform the tasks needed in synthetic genomics.
Results:
We developed BAC-browser v.2.1. It helps to design nucleotide sequences and features the following tools: generate nucleotide sequence from amino acid sequences using a codon frequency table for a specific organism, as well as visualization of restriction sites, GC composition, GC skew and secondary structure. To assemble DNA sequences, a fragmentation tool was created: regular breakdown into oligonucleotides of a certain length and breakdown into oligonucleotides with thermodynamic alignment. We demonstrate the possibility of DNA fragments assemblies designed in different modes of BAC-browser.
Conclusions:
The BAC-browser has a large number of tools for working in the field of systemic genomics and is freely available at the link with a direct link https://sysbiomed.ru/upload/BAC-browser-2.1.zip .
