Morphology of primary afferents to the spinal cord of the turtle Pseudemys scripta elegans

The morphology of primary afferents to the spinal cord of the turtle Pseudemys scripta elegans was studied by means of intra-axonal injections of horseradish peroxidase. A total of 74 collaterals arising from 34 different afferents in 22 animals was investigated. Within this sample, a division into three morphologically distinct collateral types appeared possible. Collaterals of the same parent axon could always be classified to the same type. Type A collateral arborizations could be found within area I-II and III of the spinal grey matter. The number of presynaptic boutons per collateral varied considerably. However, collaterals of the same parent axon usually possessed a similar general appearance. Type B collaterals terminated within area IV and V-VI. The general shape and number of boutons could differ considerably between collaterals of different parent fibers but also between collaterals of the same axon. Type C collaterals formed terminal arborizations in the lateral parts of areas IV, V, VI and VII-VIII and demonstrated a fair constancy in general appearance and number of presynaptic boutons. Type A collaterals are thought to be derived from fibers innervating various cutaneous receptors. Terminal arborizations of type C collaterals are fully overlapping with the dorsal dendritic trees of turtle lumbar motoneurons. It is suggested that type C collaterals form contacts with these motoneurons and arise from muscle spindle innervating afferents. The origin of type B collaterals is less clear, attractive possibilities may be found in joint and/or tendon organs.