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Updated: May 31, 2025
Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
Published on: January 7, 2019
Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a
Maria Yera1, Joshua J Wang1, Sarah X Zhang1
1Department of Ophthalmology and Ross Eye Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA.
Abstract:
Defects in retinal metabolism have been linked to the onset and progression of various retinal diseases. Herein, we provide a protocol for measuring bioenergetics in dissociated mouse retinal photoreceptors. We outline detailed instructions for obtaining morphologically intact and viable photoreceptor cells from adult mice and preparing the cells for metabolic analysis using a SeahorseXFe24 analyzer. This protocol allows a real-time assessment of mitochondrial respiration and glycolysis in retinal photoreceptors in response to genetic modifications or pathological insults in mouse models.
