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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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CRISPR: fundamental principles and implications for anaesthesia.

Alexendar R Perez1, Orestes Mavrothalassitis2, Janice S Chen3

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British Journal of Anaesthesia
|January 24, 2025
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Summary

Clustered regularly interspaced short palindromic repeats (CRISPR) gene editing is advancing medical therapies. Anaesthesiologists must understand CRISPR for patient care and its potential applications in pain management, critical illness, and oncology.

Keywords:
CRISPRchronic paincritical caregenome engineeringoncology

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Area of Science:

  • Biomedical Science
  • Genetics
  • Medical Technology

Background:

  • Clustered regularly interspaced short palindromic repeats (CRISPR)-based therapies are gaining regulatory approval globally.
  • Patients undergoing CRISPR therapy will require anaesthesiologist care, necessitating an understanding of the technology and patient profiles.
  • Anaesthesiologists' expertise may be leveraged for novel CRISPR applications.

Purpose of the Study:

  • To provide anaesthesiologists with a foundational understanding of CRISPR technology.
  • To explore the direct and indirect impacts of CRISPR on anaesthesia practice.
  • To highlight potential new roles for anaesthesiologists enabled by CRISPR.

Main Methods:

  • Review of current CRISPR technology and its medical applications.
  • Analysis of potential integration points within anaesthesiology.
  • Discussion of CRISPR's role in pain management, critical care, and oncology.

Main Results:

  • CRISPR technology presents direct applications in anaesthesia, including chronic pain and critical illness management.
  • CRISPR offers anaesthesiologists opportunities in oncology through their unique skill sets.
  • The technology may foster new domains within anaesthetic practice.

Conclusions:

  • A comprehensive understanding of CRISPR is essential for anaesthesiologists managing patients receiving gene therapy.
  • CRISPR technology holds significant potential to expand the scope of anaesthesiology.
  • Anaesthesiologists are well-positioned to contribute to and benefit from advancements in CRISPR technology.