Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk Stratification Using the Tracheostomy Early Prediction Score and the Association Between Early Tracheostomy and Mortality in Sepsis.

Respiratory care·2026
Same author

Translocator protein deficiency promotes splenomegaly with extramedullary hematopoiesis after sepsis.

Shock (Augusta, Ga.)·2026
Same author

Corrigendum to "Clinical outcomes of persistent sepsis-associated acute kidney injury in septic shock: a post-hoc analysis of a multicenter prospective cohort study" [Ann Intensive Care (2026) 100041].

Annals of intensive care·2026
Same author

Clinical outcomes of persistent sepsis-associated acute kidney injury in septic shock: a post-hoc analysis of a multicenter prospective cohort study.

Annals of intensive care·2026
Same author

Porphyromonas gingivalis-derived lipopolysaccharide confers chemotherapy resistance and migratory ability on oral cancer cells by activating toll-like receptor 4 signaling pathway.

Molecular biology reports·2026
Same author

Transgelin: a tumor suppressor candidate in neuroblastoma.

Carcinogenesis·2026

Related Experiment Video

Updated: May 31, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

850

Lactoferrin Modulates Radiation Response Under Hypoxic Conditions, Possibly Through the Regulation of ROS Production

Daitoku Murakami1,2, Takahiro Fukazawa3, Michihito Kyo1

  • 1Department of Radiation Disaster Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan.

Antioxidants (Basel, Switzerland)
|January 25, 2025
PubMed
Summary

Lactoferrin (LF) impacts radiation therapy outcomes by altering apoptosis and NRF2 signaling pathways. Its effects vary by cell type, suggesting potential roles as a radiation protector or sensitizer under hypoxia.

Keywords:
hypoxialactoferrinradiationreactive oxygen species (ROS)

More Related Videos

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

84.7K
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

17.7K

Related Experiment Videos

Last Updated: May 31, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

850
Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

84.7K
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

17.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Lactoferrin (LF), an iron-binding glycoprotein, exhibits potential anticancer activities.
  • The precise mechanisms of LF in cancer therapy, particularly radiotherapy under hypoxic conditions, remain unclear.

Purpose of the Study:

  • To elucidate the molecular mechanisms of lactoferrin's function in cells under hypoxic conditions.
  • To investigate LF's impact on cancer cell sensitivity to radiotherapy and its underlying molecular pathways.

Main Methods:

  • Cell viability assays, high-dose LF treatment, hypoxic conditions, and radiation treatment.
  • Knockdown of Hypoxia-Inducible Factor 1-Alpha (HIF1A) and assessment of Reactive Oxygen Species (ROS) activity.
  • RNA sequencing (RNA-seq), gene set enrichment analysis, quantitative RT-PCR, and Western blotting.

Main Results:

  • LF exhibited cytotoxic effects and modulated sensitivity to radiotherapy in a cell-type-specific manner under hypoxia.
  • LF's effects were linked to ROS production, apoptosis (ASC gene), and NRF2 signaling pathway regulation, with HIF1A knockdown counteracting these changes.
  • LF treatment altered gene expression profiles related to cell cycle, apoptosis, inflammation, and antioxidant signaling.

Conclusions:

  • Lactoferrin influences radiation treatment efficacy by modulating apoptosis and NRF2 signaling in a cell-specific manner under hypoxic conditions.
  • LF demonstrates potential as a modulator, acting as either a protector or sensitizer in radiation therapy.