[Changes in selected parameters of carbohydrate metabolism during short term hyperglycemia therapy in Jensen's...

Biochemical investigations of short term hyperglycemia together with tumor-lysing clostridia were undertaken in order to optimize the use of this combined anti-tumor therapy model. Repeated intraperitoneal glucose injections resulted in an increased concentration of glucose in tumor tissue which lasted several hours and paralleled the blood sugar level in tumor bearing animals. At the same time a tumor dependent deterioration in glucose tolerance appeared likely. Characteristic changes in the intratumor lactate concentration were demonstrable. Tumor acidosis lasted 4 to 7 hours. Tumor pyruvate concentrations varied little with hyperglycemia. Simultaneous blood lactate and pyruvate levels did not differ from those of controls. LDH activity as measured in the tumor and in blood serum gave no clear indication of tumor cell damage.